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Decomposition of protein tryptophan fluorescence spectra into log-normal components
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For some proteins (BSA, YADH, ALD) the profile is distinctly Multiphasic, and in many cases (all but LDH and azurin) saturation is not reached up to 300 µM ANS
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Perturbation of protein tertiary structure in frozen solutions revealed by 1-anilino-8-naphthalene sulfonate fluorescence
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Brochier,[double dagger] B
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In contrast to known protein:target binding modes, the CaM:SEF2-1mp complex shows large-scale (domain) dynamics in the bound state
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7[degrees]C (December 1999) (mean temperature [[+ or -]SE]: 15
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- Our results also suggested that the usage of resolution-enhancing techniques could provide valuable information of the step-wise unfolding of proteins
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. In a study that focused on the interaction between PLCβ^sub 2^- and Gβγ-subunits, we found, not surprisingly, that the measured steady-state dissociation constant for the two proteins was equal to the ratio of the on- and off-rates (Runnels and Scarlata, 1998)
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