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All properties characterized from simulated ensembles match predictions from rigorous field theories
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In principle, unfolded states are rather heterogeneous (26-28) and in addition the mechanisms leading to unfolded states are rather different for thermal and GndHCl-induced unfolding (1)
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However, due to the close proximity of all involved components, a new CaM:SEF2-1mp interaction is rapidly reformed, which in turn forces the CaM dimer to close again
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Entropy Calculation of HIV-1 Env gp120, its Receptor CD4, and their Complex: An Analysis of Configurational Entropy Changes upon ComplexationHsu, Shang-Te DABSTRACTThe HIV-1 gp120/CD4 interaction shows a large, unprecedented entropy/enthalpy compensation, with the capacity to fine-tune recognition over a broad range of affinity
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Table 2Means and Standard Deviations of Intrinsic and ExtrinsicMotivation Scales forParticipants Who Reported Having Gambled In the PreviousSix Months Men WomenTotal (N = 62) (N = 61)(N = 123) MSD MSD MSDIntrinsicTo Know 2
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. This limit is of interest in light of data that suggest the presence of residual structure even under strongly denaturing conditions
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. The β-mercaptocthunol was from BioRad (Hercules
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. In all cases with a typical red shift of the emission spectrum occurring during structural unfolding we observed a larger magnitude of this shift for GndHCl-induced unfolding as compared to thermal unfolding
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. All thermal unfolded states of PPA are characterized by emission spectra peaked around 335 nm
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. For this purpose, either a cooling back to room temperature or a removal of GndHCl (by dialysis) has been carried out to measure properties of the enzymes after the denaturing impact was eliminated
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. From these findings it was deduced that the solidification of water affects the tertiary structure of azurin and it was suggested that binding of the fluorescence probe could represent a general approach to monitor ice-induced strains on the protein native fold
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. In the nucleus, the signal appeared to be dominated by water, whereas other contributions were negligible
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. Early measurements (42) showed BLG has a dipole moment of a sufficient magnitude to generate significant attractive dipole-dipole interactions which are predicted to be screened by increasing ionic strength at the concentrations relevant to this study (40,43)
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. Dynamics of protein and peptide hydration
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.PRINTINGDetails of the xerographic process have been discussed elsewhere [14]
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