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. Even after increasing the metal ion concentration to as high as 30 mM, the refolding yields remain unchanged
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. However, based on the observed FRET efficiency, the two loop-carrying arms appear to be relatively far apart in that species, approximately midway between the extended and docked conformers
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. Standard histology confirmed that cells cultured in DMEM/ITS+/TGF-[beta]1 generated larger pellets than those maintained in DMEM/ 10%FCS (Figs 2 to 4)
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. At 1 M GdnHCl, one thiol group of GR became accessible to modification with 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB), whereas no changes could be detected in the spectroscopic properties (fluorescence, circular dichroism) of the protein
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. It is to be noted that observation of single resonances in NMR spectra does not necessarily mean that the exchange is fast
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. This mutation eliminates a tertiary hydrogen bonding interaction between G^sub 11^ (loop A) and A^sub 23^ (loop B) and is expected to destabilize the docked conformer (Rupert and Ferrc-D'Amare, 2001; Klostermeier and Millar, 2002)
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