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. 6, in the absence or in the presence of GdnHCl, as indicated below in Results
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. , 1989), and the enzyme concentration-dependence observed in the process suggests that the nucleotide affects the dimer (active form)/ monomer (inactive form) equilibrium of GR
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. These small changes upon binding of the effectors were also monitored by the changes in the accessibility of fluorescent residues and the trypsin digestion patterns
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. The R^sub 1^, R^sub 2^ and ^sup 15^N-{^sup 1^H} NOE data are presented in Fig
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. Gα^sub i^(GDP) can then bind to activated PLCβ^sub 2^-Gβγ and perturb the weak activating interactions without affecting the strong binding interactions as depicted in the scheme shown in Fig
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. Thus, both the appearance of tyrosine emission and the red-shift of tryptophan fluorescence indicated extensive unfolding of GR at 3 M GdnHCl
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. The tetrameric band at 55 kD originates from the amount of unfolded monomers that have not yet been misfolded/misassembled and can refold instead of forming aggregates during the pH jump (from pH 2 to pH 7
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