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. Considering the commercial importance of aminoacylase, the results herein might facilitate the optimization of the conditions for the industrial use of ACYI
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. The best fitting results were obtained according to the least root mean square criterion
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. Conversely, gSBA showed the same hydrodynamic radii until 3
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. Similarly, there does not appear to be any effect of the extent of glycosylation on the kinetic characteristics of either Drosophila (63) or human (64) AChE
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. ^sup 15^N chemical shifts were indirectly referenced using the gyromagnetic ratio of ^sup 15^N/^sup 1^H (18)
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. Protein folding and misfolding
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. An example is given in Fig
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. It should be noted, though, that entropies originating from different degrees of freedom need not be additive and that a decomposition will neglect correlation of motions along different degrees of freedom
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. The proteins were redissolved in D^sub 2^O and centrifuged at 6000 × g for 10 min before use with a final pD of 8
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.[FIGURE 2 OMITTED]The Phagocytic SynapseBecause the interaction between an apoptotic cell and a phagocyte involves an array of receptors and bridging molecules, it has been termed a phagocytic synapse
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. An estimate of the number of binding sites requires knowledge of the average fluorescence quantum yield (φ^sub F^) of ANS bound to azurin
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.A challenge faced by all these models is that the most successful search for inherent features of tertiary structure that correlate with folding rates has found that the topological measure of "contact order" is far more closely related than, for example, molecular weight itself in the case of "two-state" folders (Plaxco et al
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. The two species could be distinguished by the placement of the first process of the premaxilla, which is perpendicular to the premaxilla in the Argentine sandperch, and backinclined in the Brazilian sandperch, drawing an acute angle with the premaxilla (Herrera and Cousseau, 1996)
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. However, when a polymer-solvent system undergoes LLPS prior to crystallization in a certain range of polymer concentration (see Figure 1), the crystallization curve is modified
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. Proceedings of the Pennsylvania Academy of Science 61:133-137
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