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We attempted, by docking of the EIN^sup sc^ and EIC^sup sc^ domains, to model the whole structure of EI^sup sc^ trying to keep the active-site histidine (in the EIN^sup sc^ domain) close to the PEP binding site (in the EIC^sup sc^ domain) loan student uk .
An excitation wavelength of 280 nm (bandwidth 3 nm for the Perkin-Elmer and 10 nm for the Shimadzu spectrometer) was applied while emission spectra were recorded in the range between 300-450 nm (bandwidth 5 nm and 10 nm for the Perkin-Elmer and the Shimadzu spectrometer, respectively) loan student uk .
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Calculations (21) show that if a longer ranged attraction is added on to the AHS model, although the percolation line does not move, the effective stickiness varies such that the liquid-liquid coexistence line is no longer below the percolation line, a potential explanation for the absence of particle gelation loan student uk .
33 K/min is observed above 80°C, ~10°C higher than the onset of the corresponding CD transition loan student uk .
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