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. 8, both plots revealed that there are two linear segments that deviated at a temperature of around 68°C, and each straight line reflected a two-state process
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. The interactions between the attached glycans and the protein were assessed using the online LPC server (24)
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. This work was supported by a grant from the Dept
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. Beyond local length scales, chain properties follow well-defined power laws that are expected for generic polymers in the EV limit
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. Experiments were only carried out at 280 nm
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. 1°C/min reveals for all protein concentrations λ^sub max^-values around 345 nm for the unfolded state
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. 5a, there is no difference in maximum wetting forces for Sn-Pb solder at 255°C and 275°C, whereas Sn-Ag-Cu-Bi solder notices the increase in maximum wetting force values from 0
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. All three probes bind with similar affinity to these oligomers with DCVJ, indicating the strongest affinity showing I^sub 50^ values of 1
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. These barriers can be overcome by proteolytic or lipolytic enzymes such as LCAT, phospholipid transfer protein, or cholesterol ester transfer protein
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. Under these experimental conditions, no prominent qualitative changes in the elementary morphological folds were observed at times of incubation exceeding 1 h
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. © 2005 by the Biophysical Society0006-3495/05/01/172/12 $2
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. brasilianush The abundance and distribution of P
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. We also thank Atila Gosztonyi, Raul Gonzalez, and two anonymous reviewers for providing useful comments on the manuscript
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.00199 kcal/mol-K is the ideal gas constant and T = 298 K is the simulation temperature
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. The 400-MHz data were analyzed with ^sup 1^H and ^sup 15^N diameters of 6
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.Here, p^sub A^ and p^sub B^ are the populations of the states A and B, Δ^sub ex^ = Ω^sub A^ - Ω^sub B^ is the chemical shift difference between the two states, k^sub ex^ = k^sub A[arrow right]B^ / p^sub B^ = k^sub B[arrow right]A^ / p^sub A^ is the rate constant for the exchange process, δ is the delay of the δ-180°-δ CPMG block and ω^sub I^ is the frequency of nucleus I
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., 2003a,b; Fang et al
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