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Steady-state and quenching experiments were acquired at 298 K
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all histidine residues were solvent-exposed: and
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Resting and cytokine-stimulated human small airway epithelial cells recognize and engulf apoptotic eosinophils
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A similar difference in emission peak maxima between thermal and GndHCl-induced unfolding was also observed for other proteins (see for example (10,21))
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At this pH, the osmotic pressure is weakly dependent upon salt concentration, increasing at high salt concentrations
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0 no salt), and soluble misfolded oligomeric state (pH 2
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5M ammonium hydroxide (9:7:2, v/v) under nonreducing conditions
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Similar unfolding below 25°C was detected in the earlier studies of human lipid-free apoA-2 at much higher protein concentration (>0
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After 48-h incubation, the disk reconstitution appears incomplete and a sharp lipid transition of reduced amplitude is detected (Fig
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The folding of RNA is generally coupled to its biological function, and this is illustrated well by the hairpin ribozyme, a small catalytic RNA with both endonuclease and ligase activities
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