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. The results in Fig
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. As a consequence the observed emission spectra of individual proteins (and of their corresponding conformational states) do not even vary with respect to λ^sub max^ but also can show different spectral shapes and widths (7,8)
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. To examine the reason for this factor, we compared T^sub 2^, the spin-spin relaxation times, of the methylene peak at 1
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. Hence, with reasonable SNR, even one or two millimolar concentrations appear to be detectable noninvasively on a cellular level, rendering localized spectroscopy with Xenopus oocytes an attractive methodology to noninvasively determine the permeability of a cell for specific drugs, especially since the membrane of amphibian oocytes frequently serves as a broader membrane model (45-49)
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. Of course, it is not impossible to achieve the dimensional reduction we have outlined by using fully native, rather than nonnative interactions
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. As an illustration, conformational entropy buildup curves as a function of block size with error bars derived from the block averaging procedure are presented for gp120 atom sets in Fig
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. DISCUSSIONComparison of spFRET histograms and ensemble trFRET measurementsOur spFRET results for the wild-type ribozymes show the presence of extended (undocked) and docked conformations, with relative populations that are strongly dependent on the Mg^sup 2+^ concentration
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