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. The interactions between the attached glycans and the protein were assessed using the online LPC server (24)
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. An estimation of the T^sub m^ of EIC^sup sc^, based on the fluorescence results (Fig
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. Processive enzymes, when sliding along their targets (50), have the closest similarity to CaM:SEF2-1mp binding
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. Additionally, PLCβ^sub 2^ and β^sub 3^ can independently be activated by Gβγ-subunits as well
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. Data analysisConversion of the apparent K(d) obtained on membrane surfaces to the K(d) that would be obtained if the proteins were freely diffusing has been previously treated by assuming that the proteins are confined to interact within a reduced surface volume (ν) that is equal to the surface area of the membrane multiplied by the distance from the membrane surface into the aqueous phase where the proteins interact, which is taken to be 50 nm
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. The chains are connected by two disulfide bridges: A7-B7 and A20-B19, whereas a third bridge binds cysteine residues 6 and 11 of the A-chain (e
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. Put another way, the vast majority of the space covered by the energy landscape must actually be flat (on a scale of k^sub B^T) rather than funneled
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. However, we note there was no significant effect of Advice/No-Advice, F(l, 14) = l
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. The I [arrow right] A transition, which was referred to as the dominant unfolding process above, began at around 70°C and the midpoint of the transition (T^sub 1/2^) occurred at ~84°C (see Table 1)
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. Study of the dynamics of the complex can give insights into this novel type of CaM:target interaction
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. Electron microscopy, gel electrophoresis, and differential scanning calorimetry show that such fusion involves lipid vesicle formation and dissociation of monomolecular lipid-poor protein
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