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"It's like pulling a thimble two feet through a room, then counting the dust motes caught in the thimble and trying to estimate the quantity of dust in the whole room," says John Boreman, director of the research center in Woods Hole
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The enzyme concentration was determined by measuring the absorbance at 280 nm and using the absorption coefficient A^sup %^^sub 1cm^ = 13
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These observations in the IR spectra coincided with the deduction based on DSC results that the thermal unfolding of ACYI is an irreversible process accompanied with protein aggregation
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It is on the basis of this invariance to "spatial dilatations" (39) that polymers in the EV limit are said to he rcnormalizable entities
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Folding rates for two-state proteins show statistically significant correlation with native-state contact order (104)
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INTRODUCTION The bacterial phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) is a multiprotein complex system that phosphorylates and, concomitantly, transports carbohydrates across the membrane into the cell (1)
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HPr^sup sc^, in contrast to the HPrs in Gram-negative bacteria and low-G + C Gram-positive bacteria (1), is not involved in general carbon regulation (20,21)
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The differences among the modeled structure and that of EIC^sup tt^ in the hydrogen-bond pattern, and the van dor Waals contacts in the dimerization interface could explain those changes
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We thus did our experiments with a much higher rf bandwidth (11 kHz) and a shorter rf pulse (0
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The similar T^sub 2^ values of the animal and vegetal methylene peaks imply that molecular motion occurs at the same timescale in both compartments and cannot account for the linewidth difference
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The sliding process is likely to occur on a nanosecond timescale, although CaM:SEF2-lmp interactions may slow down this process in some instances
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These include, but are not restricted to, native and near-native hydrophobic side-chain interactions
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Unfortunately, not all individuals caught during the cruises were examined by us; therefore, to avoid biases caused by identification errors, the posttransition juveniles of both species were considered as a group
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The parameters, such as polymer concentration and cooling rates for phase separation of polyethylene, were quite different from those used in our study to produce IPP and EVA toners
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The peak positions for the SL 2WJ and 3WJ constructs closely follow the trends observed for the undocked peak with the corresponding WT constructs, confirming that removal of the loop on arm A provides appropriate models for the undocked states of the WT ribozymes
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5 million for Type 1 RP and $22
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6 billion for the commercial sector
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* Push--A tie between the dealer and player in which no money changes hands; a standoff
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. The changes of thermodynamic parameters (enthalpy change ((Delta)H), entropy change ((Delta)S) and heat capacity change ((delta)C^sub p^)) corresponding to the transitions from N to MG (molten globule state) or MG to U (unfolded state), were summarized in the previous report (Nishii et al
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.The second hypothesis is that genes controlling the development of unnecessary structures become effectively neutral
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.Identification of nanosecond-timescale internal motionThe presence of nanosecond-timescale motion can be directly identified from a plot of the normalized ratio of R^sub 1^ values measured at two magnetic fields (RT^sup n^^sub 1^) versus NOE (22)
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., at the tips of the N- and C-terminal domains, ii), close to the hinge region connecting the N- and C-terminal domains, iii), close to the hydrophobic pockets in the N- and C-terminal domains where the target binds (see Fig
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