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. and those made by us, we may conclude that rSBA and gSBA are similar in all other respects (secondary and tertiary structure, size, and function) except for glycosylation (as shown by the difference in the matrix-assisted laser desorption ionization mass spectra in Supplementary Material, Fig
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. Steady-state ANS binding ANS binding was detected by collecting fluorescence spectra at different pH in the presence of 100 µM of dye
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. This shows that liquid-liquid separation occurs in a region of the phase diagram which is metastable with respect to solid-liquid phase separation
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. 1, the osmotic pressure data indicate that the dominant interaction between the protein particles is repulsive
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. Abbreviations used: PLC, mammalian phosphoinositide-specific phospholipase C; PI(4,5)P^sub 2^, phosphatidylinositol 4,5- bisphosphate; POPC, 1-palmitoyl-2-oleoylphosphatidylcholine; POPS, 1-palmitoyl-2-oleoylphosphatidylserine; PE, 1-palmitoyl-2-oleoylphosphatidylethanolamine; PH, pleckstrin homology; DNS-PE, dansyl phosphadtiylethanolamine; FRET, fluorescence resonance energy transfer
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. PROCEDURE FOR PRODUCING TONERThe effects of several polymer and process parameters on particle size were investigated (see Table III) and a suitable set of parameters of the required particle size was chosen
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. We evaluated this expression at the average value of t in the post-Type 1 RP introduction period and tested it with Student's t-test
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. We believe that this result provides a physical basis for the robustness of native-state topology in protein-folding studies
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. 5), must be at a higher free energy than the native unfolded state, C (Fig
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. Thus, the residues with significant exchange broadening can be placed into two groups: those located in helices with a relatively high activation barrier, and those located in loops with a relatively low activation barrier
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.Nuclei that are influenced by chemical exchange have been shown to correlate with residues known to be critical for protein interactions and enzymatic activity (39,40, 41,42,43,44)
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.1, the osmotic pressure data indicate that the dominant interaction between the protein particles is repulsive
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. An approach by reassembly techniques
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