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6 shows the derivative plot of the respective thermal melts
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We use our results to resolve between conflicting proposals for structure in ensembles for highly denatured states
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This energy difference is used with the Metropolis criterion (61) to accept or reject the proposed move
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00199 kcal/mol-K is the ideal gas constant and T = 298 K is the simulation temperature
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That this is indeed the case is shown by comparing cavity statistics in the EV limit to values obtained for freely rotating chains
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Thermodynamics and kinetics of non-native interactions in protein folding: a single point mutant significantly stabilizes the N-terminal domain of L9 by modulating non-native interactions in the denatured state
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When ANS binds to solvent-exposed hydrophobia patches, its quantum yield is enhanced and the maximum of emission is shifted from 520 nm to 480 nm (39,40)
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Recently, a study conducted in our laboratory investigated the possibility to unveil potential alterations of the protein tertiary structure in ice from binding of ANS (12)
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In all fluorescence experiments the samples were prepared from ANS and protein stocks to a final constant protein concentration of 2 µM in 2 mM Hepes buffer, pH 7
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It is a homodimeric peptide formed via a disulfide bridge between two cysteine residues located at position 19 of the two 21-residue-long peptide strands
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1 was observed at shear rates above 50 s^sup -1^
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The simplest explanation is that GDP-bound Gα^sub s^-subunits bind to Gβγ, but not necessarily at its primary interaction site
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In distribution centers, lighting in cold rooms is often on for long periods, exposing such containers to offending UV light
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For histogram simulations of the SL 4WJ at 10 mM Mg^sup 2+^, the state of the ribozyme system was allowed to stochastically switch between the extended undocked and quasi-docked states according to the assumed forward and reverse rate constants (see Fig
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126 -2,895 23,228OpiatesAverage monthly effect 492 <
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