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Predissociated Dimers and Molten Globule Monomers in the Equilibrium Unfolding of Yeast Glutathione ReductaseLouzada, Paulo RobertoABSTRACTThe equilibrium unfolding of dimeric yeast glutathione reductase (GR) by guanidine hydrochloride (GdnHCl) was investigated
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Both of these states are characterized by expanded hydrodynamic radii, loosening of tertiary structure, and preservation of secondary structure
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1°C/min) gives rise to less pronounced aggregation during the thermal unfolding transition as compared to higher heating rates; and (2), the lowest protein concentration (7 µg/ml) reveals a less-pronounced aggregation also for a higher heating rate (1°C/min), whereas higher protein concentrations studied with the higher heating rate exhibit more pronounced aggregation
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Using polyethylene glycol solutions as a model of a crowded cell environment, we studied DNA compaction by alkali metal salts LiCl, NaCl, KCl, RbCl, and CsCl, and found that all of these monocations promote DNA compaction
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thanks Japan International Science and Technology Exchange Center for fellowship No
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A schematic representation of the TTR misfolding reaction from the unfolded state through the A-state is presented in Fig
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Additionally, PLCβ^sub 2^ and β^sub 3^ can independently be activated by Gβγ-subunits as well
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Particle diameters were assessed from comparison with protein markers with known Stokes radii and were confirmed by EM measurements
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Observations by optical microscopy (OM) reveal agglomerates of pigment in the particles [14]
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When comparing the melting rates of the three polymers, the melting temperatures should be considered
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Further research is necessary to explore the controlling mechanisms of differential reproductive success with age and to determine how best to incorporate these findings into stock assessment models
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Fecundity of the widow rockfish, Sebastes entomelas, off the coast of Oregon
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As a result, the specifically-docked conformation exchanges only slowly with extended conformations, resulting in a relatively narrow and symmetric peak and an absence of peak shifts in the FRET efficiency histogram
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An index by primary indication would be a useful addition to the next edition
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