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To characterize in more detail the structural changes induced by GdnHCl, we have investigated the accessibility of thiol groups of GR (which contains six Cys residues per subunit) to modification with DTNB (Fig
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Protein measurement with the folin phenol reagent
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Chánez-Cárdenas, M
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The S^sup 2^^sub s^ was either kept at a uniform constant value (M2I) or optimized together with τ^sub if^ and S^sup 2^^sub f^ (M2II)
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0 the virial coefficient remained negative as also found by Schaink and Smit (40) using membrane osmometry (mixed BLG A and B) at pH 5
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However, repeating this study for solubilized proteins that were freshly dialyzed to remove detergent gave an apparent K^sub d^ only 10-fold weaker than predicted, suggesting that we may be not be viewing a simple protein-protein association (K^sub d^ ~ 70 nM; Fig
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2) than those with apoA-1 (data not shown)
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The solutions appeared visually clear for temperatures at or above 130°C and a dissolving time of 2 hours, but small nuclei below the visible range may still have remained
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However, the dissolving temperature was changed
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Mg^sup 2+^-dependent folding of a large ribozyme without kinetic traps
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