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Unfolding of GR by GdnHCl was further characterized by intrinsic fluorescence anisotropy measurements (Fig
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5 C to avoid the possible artifacts caused by large band shifts accompanied with slight intensity changes in this temperature range (see also Fig
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Experiments that only detect close spatial contacts will be interpreted as providing evidence of long-range "residual" structure in the denatured state (94-98)
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Structural interpretation of the nanosecond-timescale internal motionThe dimeric CaM:SEF2-1mp complex can in principle show internal modes of motion involving either the individual N- and C- terminal domains and/or the CaM monomers as a whole
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The tetrameric band at 55 kD originates from the amount of unfolded monomers that have not yet been misfolded/misassembled and can refold instead of forming aggregates during the pH jump (from pH 2 to pH 7
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The time course of the protein unfolding, which was triggered by a rapid increase in the Gdm HCl concentration from 0 to 1-6 M, was monitored by CD at 222 nm (Fig
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It is also possible that "kinetic traps" arise not just from low-energy intermediate states, but from intermediate diffusive subspaces of higher dimension than two, for which the control of dimensionality has been incomplete
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The mechanism appears to center around casein derivatives in cheese including glycomacropeptide
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During those months without reproductive activity, late spring through early fall, it was difficult to distinguish between immature and mature-resting ovaries
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Fecundity of the widow rockfish, Sebastes entomelas, off the coast of Oregon
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It calls upon domains of expertise largely foreign to workers' compensation professionals
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.The backbone structures of the CaM N- and C-terminal domains within the CaM:SEF2-1mp complex are expected to be very similar to those in other CaM structures
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