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. Interestingly, the difference in T^sub m^ of the two forms is ~26°C, and this estimates the extent of stability imparted by the oligosaccharide chains to the stability of gSBA
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. On the other hand, the antigen recognized by Mab217 has not yet been characterized and could still act as an efferocytosis receptor, possibly through the recognition of PS
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. Besides the shift of transition temperatures caused by different heating rates, pronounced differences in the λ^sub max^-values for the unfolded states are visible in Fig
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.As expected from Fig
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. PLCβ does not have these modifications and is aqueous soluble; however, it binds strongly to membrane surfaces with little specifically (Runnels et al
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. 1 C), yet it shows hysteresis and scan rate effects characteristic of thermodynamically irreversible reactions, as indicated by the CD and light-scattering data in Fig
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. Possible shrinkage was not considered in the measurements
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. In each step, chloride salts of appropriate cations (Ca, Fe, and Mg) were dissolved in deoxygenated, CO2-saturated water
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.Folding of single-loop ribozyme constructsThe results described in the preceding section suggest that Mg^sup 2+^-induced conformational changes within the helical junction region may control the approach of loops A and B during ribozyme folding
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