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Experiments were carried out at room temperature in 30 mM HEPES, at pH 7
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1), and undergoes a progressive red-shift with increasing concentrations of GdnHCl
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These data reveal an increased flexibility of native HuBChE relative to its soman-inhibited counterpart above 334 K (61°C)
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Palmitoyl groups are typically attached to Cys side chains, and these linkages can be reversed by the addition of reducing agents
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, before the onset of the second calorimetric transition, is fully reversible and leads to no detectable changes in the subsequent DSC scans (data not shown)
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