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Since no crystal structure of ACYI is available, all four of the components (A and S, I, II, and III) of Trp fluorescence defined previously (33,34) were included in the curve-fitting process
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6^, δ) for Fyn SH3 domain
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In contrast all thermal unfolded states (with the exception of those obtained from PPA) exhibit λ^sub max^ values around 340 nm, which correspond to much smaller red shifts upon unfolding
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A sample of ~200 mg DmAChE was dialyzed against, and then lyophilized from 0
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Despite these features the overall interaction is highly specific with nanomolar binding strength (16)
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The R^sub 1^, R^sub 2^ and ^sup 15^N-{^sup 1^H} NOE data are presented in Fig
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The effect of high pressure on the membrane association of different types of peripherally bound and integral membrane proteins have been reported, and their pressure behavior is variable (e
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The prolonged 10-min incubation at 60°C promotes further elongation of the fibers even up to 2
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The two fibers marked with the green and blue arrows are of the comparable height of 6
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Biomolecular Simulation: The GROMOS96 Manual and User Guide
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, unequal elevations) which necessitated that the data be fitted with a parallel-line multiple linear regression model (Fig
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Appropriate blocking steps were included for endogenous peroxide
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J Orthop Res 1990:8:266-75
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5] t, t [greater than or equal to] November 1995 (3)Under the null hypothesis that Type 1 RP did not affect quantity, [[gamma]
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.6, in the absence or in the presence of GdnHCl, as indicated below in Results
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. The statistical proof of discreteness of tryptophan classes in proteins
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. All the experiments were done in a Jobin Yvon Horiba fluorometer (Jobin Yvon Spex, Cedex, France) in a 1-cm water- jacketed cell using a protein concentration of 1 µM and ANS concentration of 50 µM
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. and those made by us, we may conclude that rSBA and gSBA are similar in all other respects (secondary and tertiary structure, size, and function) except for glycosylation (as shown by the difference in the matrix-assisted laser desorption ionization mass spectra in Supplementary Material, Fig
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. 9, which plots the probabilities, P^sub oa^(r = U), of finding cavities of different sizes at the ensemble-averaged center-of-mass as a function of cavity radius a
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. The reversibility of thermal transitions was tested by recording a new scan alter cooling the thermally denatured sample to 298 K, and comparing the thermal denaturation curve with that obtained in the first scan
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. As a first step in this direction, we measured the thermal unfolding transition of α-amylases in the presence of NDSB, which is known to be an effective agent to prevent aggregation of thermal unfolded proteins (32-34)
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. They are clustered in the CaM:SEF2-1mp binding interface, the CaM:CaM dimer interface, and in the flexible helix connecting the CaM N- and C-terminal domains, and have similar exchange times (~50 µs), suggesting a cooperative mechanism probably caused by protein:protein interactions
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. ^sup 15^N-{^sup 1^H} NOE values were determined from the ratios of peak volumes recorded in presence and absence of proton saturation
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.12 ml g^sup -1^) gives better agreement at higher protein concentrations (Fig
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