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. Preliminary Fourier transform infrared spectroscopy (FTlR) and circular dichroism (CD) thermal denaturations at physiological pH on the EI enzyme of S
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. The joint position of the changes in the two adjacent lines was around 68°C, suggesting the appearance of an intermediate at ~68°C
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. As a result, for each sequence, we generated an ensemble consisting of 10^sup 4^ uncorrelated conformations
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. the DSC experiments with EI^sup sc^ did not show two transitions
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.) the ensemble of unfolded states is characterized by diverse structural and conformational properties
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. Smaller heating rates are accompanied by less-pronounced aggregation
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. With respect to corresponding CD spectra we observed for both types of samples (i
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. In agreement with the previous report (12) for WT azurin, the intensity is enhanced ~7-fold and the spectrum is blue shifted by 13 nm relative to the ANS control
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. For prolate ellipsoids of this aspect ratio, the maximum packing fraction is ~0
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. Bis-ANS shows very strong fluorescence in response to all conformational states of TTR, with the strongest signal emanating from the rapidly formed monomeric A-state upon NaCl addition
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