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. The data support the presence of at least two intermediate states in the equilibrium unfolding of GR by GdnHCl
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. Although gSBA unfolded via a two-state process without the formation of any unfolding intermedialcs, the denaturation profile of rSBA was poputated with an intermediate (Fig
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. Experiments were recorded between 300 and 400 nm
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. The dynamics is similar to that found for the "wraparound" binding mode in some aspects, e
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. Size and morphology of special aggregates and protofibrilsAliquots from a TTR aggregation reaction solution taken after 30 min, 2 h, 24 h, and 430 h were investigated using transmission electron microscopy (Fig
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. In preflexion and flexion larvae, the finfold was present and it was gradually lost as the true fins developed
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. For a molecule existing in two different states but with similar atom-positional fluctuations, similar configurational entropies are expected for the two states
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. We conjecture that holding at 160°C destroyed undissolved nuclei in the solution, and when the solution was cooled, fewer but larger particles were formed
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. RNA conformation and folding studied with fluorescence resonance energy transfer
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. RESULTS In this work, we focus on two-state proteins because the hypothesis is that only two well-defined macrostates-native and highly denatured states-are accessible to these systems (67-70)
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. Protein was extensively dialyzed against 2 L of the working buffer (5 mM HEPES buffer containing 10 mM NaCl and 2 mM β-mercaptoethanol, pH 7) at 278 K
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. In EI^sup sc^, a single peak was observed in CD and DSC experiments, but in the pH range where the thermal transitions could be followed (pH 7-9), two different peaks were not observed; further, the T^sub m^ of the sole transition observed was pH-indcpendent between pH 7 and 9 (Fig
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. The spatial resolution of MRI typically ranges between millimeters and hundreds of microns (4,5), whereas MRS-which generates signal from nonwater protons whose concentrations fall far below those of the water protons used in MRI-requires voxel sizes of tens of millimeters for humans to a few millimeters for smaller animals like rodents (6,7)
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. In a second experiment, we compared dimeric DmAChE and tetrameric HuBChE
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. Upon Ca^sup 2+^ binding, a conformational change enables it to interact with over 100 different target proteins, including transcription factors (for reviews, see Van Eldik and Watterson and others (6,7))
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. Experimentally these states have been principally observed in colloidal systems although it has been shown that there are strong analogies between the behavior of colloidal and concentrated globular protein solutions (2)
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. Although the data from such measurements are noisy due to the short data collection time (2 min), we found good correlations with the aggregation kinetics of ANS binding using steady-state fluorescence (Fig
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. The scan rate was tuned proportionally to the area scanned (from 500 nm × 500 nm to 10 µm × 10 µm) and was kept within the 0
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. An additional example of tuned nonnative interactions guiding a folding pathway occurs in the rather larger Phage 22 Tailspike protein (Robinson and King, 1997), where a nonnative disulphide bond controls the folding search
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. The southernmost limit where larvae were collected was within San Jorge Gulf, which was surveyed in late March (fall)
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. These intrinsic features of RNA folding reactions can be obscured due to the averaging inherent in conventional ensemble experiments
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.9 at high Mg^sup 2+^ (Fig
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.Table 4: Effect of RP on Pharmacare NSAID Expenditures NSAIDs Estimatep-ValueEffect of Type 1 RPCumulative effect over all months-$7,506,214<
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.51); the mean mental component (MCS12) HRQOL score was 38
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