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Oligomerization and aggregation of bovine pancreatic ribonuclease A: characteristic events observed by FTIR spectroscopy
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First, we present a detailed description of the methods used in our work
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Measuring unfolding of proteins in the presence of denaturant using fluorescence correlation spectroscopy
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Thermal denaturation measurements Thermal denaturation experiments were carried out with a scanning rate of 50 K/h
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Solvent-accessibility of aromatic residues (acrylamide quenching experiments) Quenching experiments in the presence of the effectors yielded quenching constants in the presence of Mg^sup 2+^ (2
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and would alter the fluorescence of Trp461 (Fig
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5 b, but was also observed for all other α-amylases which otherwise exhibit aggregation upon thermal unfolding
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Our gradient coil capacity allows still higher gradient strengths, but these cause larger eddy currents and possibly also signal distortions in MRS experiments
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8) is structurally and catalytically very similar to AChE (21)
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After dipping into the solders, the samples were mounted with resin, cured at room temperature, mechanically ground, and then polished in order to obtain the cross sections of the solder/substrate interfaces
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The minimum torque limit was 2 × 10^sup -6^ Nm
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To estimate errors, several independent simulations could be performed and compared
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The immunoglobulin subclass of the monoclonal antibodies was determined by observing the positions of bands that appeared on the stick
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In those 2 studies, however, the staining was entirely cytoplasmic, whereas the staining observed in our cases was mainly located on the cell membrane, with only some marginal cytoplasmic immunoreactivity
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; the BC Ministry of Health; and the Drug Information Association is acknowledged
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