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Mostly a conventional federal bureaucracy, NOAA also conducts the scientific research upon which all regulations are purportedly based, through its six research centers such as those in Woods Hole, Massachusetts, and LaJolla, California
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This article deals with the unfolding reactions of the nonglycosylated recombinant form of the protein rSBA and its comparison with the glycosylated counterpart gSBA
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The International Milk Genomics Consortium (IMGC) represents a forum for this discussion and supports an Internet-based data management system where members upload and download
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Although in a system as complex as the PTS, it is likely that more than one form of regulation must exist, the existence of the monomer-dimer equilibrium and the non-sugar specificity of EI make this protein an excellent target to control different cellular functions (such as transport and chemotaxis) (5) and to understand the protein-protein interactions in the PTS
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The other major change in a fluorescence signal upon conformational changes of the protein is related to solvent relaxation, which determines the magnitude of the stokes shift
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The stability of these mutants is ~3 kcal/mole lower relatively to the WT (20-22), and the internal flexibility of the polypeptide is considerably larger, particularly with I7S and F110S (18)
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One micrometer resolution NMR microscopy
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In the case of Ni substrate, a binary Ni-Sn IMC was detected for Sn-Pb solder, whereas a ternary Cu-Ni-Sn IMC was found for Sn-Ag-Cu-Bi solder, as shown in Fig
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The fluorescence intensity was registered by integration of the spectra to avoid adverse effects by spectral shifts
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We employed the DCVJ probe to investigate its potential use for recognition of misfolded aggregates formed from TTR
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Evidently increasing viscosity increases the quantum yield of DCVJ (Fig
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Pseudopercis semifasciata and Pinguipes brasilianus (the other sympatric species of pinguipedid fishes) posttransition juveniles were distinguished by their head shape, pigmentation pattern, and by the number of spines of the dorsal fin (five in P
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By combining the trajectories of the free and complex forms, further insight into the conformational sampling was extracted
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In the combined trajectory analysis (Fig
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2] at room temperature for 15 minutes
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Hence, the 4WJ ribozyme is unique in its ability to fold into a structure with arms A and B in close proximity, similar to the native ribozyme, even in the absence of specific loop-loop docking interactions
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The patient was discharged home the same day
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