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In native proteins containing both tyrosine and tryptophan residues, tyrosine emission is not commonly observed due to efficient energy transfer from tyrosine to neighboring tryptophan residues (Lakowicz, 1983) ohio small business loan .
The fluorescence spectral centers of mass showed a marked (~13-14 nm) red-shift between 1 M and 3 M GdnHCl (Fig ohio small business loan .
This leads us to further understanding of intermediate protein structures in the unfolding process ohio small business loan .
Intrinsic fluorescence spectroscopy The samples for the fluorescence measurements were prepared by dissolved ACYI in 30 mM Tris-HCl (pH 7 ohio small business loan .
1628 cm^sup -1^ or the fluorescence intensity at 320 nm vs ohio small business loan .
© 2007 by the Biophysical Society 0006-3495/07/01/578/10 $2 ohio small business loan .
We carried out measurements at different protein concentrations either by CD and fluorescence in the concentration range 4-8 µM at pH 7 ohio small business loan .
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