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quantifying the interactions between unfolded molecules at high concentrations (29), assessing the presence of residual interactions between hydrophobic as well as charged groups (119-122)
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Oxford University Press, New York
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Protein was extensively dialyzed against 2 L of the working buffer (5 mM HEPES buffer containing 10 mM NaCl and 2 mM β-mercaptoethanol, pH 7) at 278 K
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At any of the explored pH, the percentages of β-hairpin (at 1693 cm^sup -1^) turns/loops/(0, π) β-sheet (at 1679 cm^sup -1^) and antiparallel β-sheet (at 1633 cm^sup -1^) remained essentially unaltered
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5 K for Mg^sup 2+^, -1
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These points and the liquid-glass transition boundary have been added to the nonequilibrium phase diagram (Fig
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In the coexistence region (Fig
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As a result, the small size of a hydrated cation provides effective interaction with DNA due to higher Coulomb electrostatic potential (Rouzina and Bloomfield, 1996) and the possibility of being incorporated into the DNA minor groove (Bartenev et al
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Interestingly, in these two samples (1 h and 1 week incubation at 70°C) the fibers do not criss-cross
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Core gp120 is defined as the construct present in the crystal structure with the truncated V1-V3 loops and N- and C-termini
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The more protracted the reproductive period, the greater the likelihood that some spawning will occur during conditions favorable for larval survival (Lambert, 1990)
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Docking of the two loops is also observed in simpler ribozyme constructs comprised of two- or three-way helical junctions (2WJ and 3WJ, respectively), although much higher Mg^sup 2+^ concentrations are required (N
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