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. Protein concentration was 4 µM
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. A further aspect of nonequilibrium behavior exhibited by solutions of proteins with short-ranged attractive interactions is metastable liquid-liquid separation (11,12)
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. Thus, the initial state in the first jump was intact disks, and in the second jump it was a mixture of protein-containing vesicles and lipid-poor protein
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. In apoA-2:DMPC complexes, the repeatability of the high-temperature transition that involves unfolding of lipidated apoA-2 (Fig
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. 2 B), where a subspace of the conformational space of the free molecule is sampled in the complex state
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. In our EV model, there is only one free parameter, namely the exponent n
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. If the move is accepted, we set U = U', return to step 2, and iterate until convergence
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. Comparing enzymes of the same family would certainly be of value in this context
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. The interaction was not significant, F (1,140) =
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