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. Thermodynamic study of the apomyoglobin structure
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. MATERIALS AND METHODS Materials Tris (hydroxymethyl) aminomethane buffer, [N-[2-hydroxyethyl]piperazine-N'-[2-ethanesulphonic acid]] (HEPES), and guanidinium hydrochloride were from Sigma Chemical (St
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. In an attempt to correlate affinities and n values to specific aspects of the macromolecule, we observe that all mutants are less stable than the WT by a comparable amount, the denaturation-free energy change, ΔG° at 20°C, decreasing from 6-7 to ~3 kcal/mole (20,27)
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. MATERIALS AND METHODS Materials Tris (hydroxymethyl) aminomethane buffer, [N-[2-hydroxyethyl]piperazine-N'-[2-ethanesulphonic acid]] (HEPES), and guanidinium hydrochloride were from Sigma Chemical (St
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