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. Interestingly, when GR was incubated in the presence of 3 M GdnHCl the area of the 120 kDa peak decreased markedly, and a second peak corresponding to a molecular weight of ~60 kDa appeared
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. Solvent stabilization by salting-out ions is a consequence of a decrease in free energy of the native state, due to both entropic and enthalpic contributions; in particular, the effect on hydration water is expected to be mainly entropic, whereas the observed decrease in protein MSDs (44) indicates a tighter packing of the apolar residues in the core of the protein as suggested for hydrophobic stabilization by calorimetry experiments (59)
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. An ion with a smaller radius corresponds to stronger hydration, and the hydrated radius sequence for monocations is Li+ > Na+ > K+ > Rb+ > Cs+
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. The fluorescence intensity of the different probes in the presence of the unfolded monomer state is shown in Fig
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. For example, the apparent melting temperatures inferred from the CD data recorded at 80 K/h are T^sub m,A-1^ = 91°C, T^sub m,A-2^ = 84°C, and T^sub m,C-1^ = 72°C
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. Calculation of the absolute molecular entropy would require a complete translational and rotational sampling
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