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A figure presenting the matrix-assisted laser desorption ionization mass spectra of the glycosylated and nonglycosylated form is shown in the Supplementary Material
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This work was supported by a grant from the Dept
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This concern seems to be substantiated by in vitro studies (34) showing the proinflammatory effect of lysed neutrophils in an elastase-dependent manner
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A further aspect of nonequilibrium behavior exhibited by solutions of proteins with short-ranged attractive interactions is metastable liquid-liquid separation (11,12)
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5 ns, resulting in a final increase of 0
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To isolate the contribution of the helical junctions to tertiary folding, we also studied the corresponding ribozyme constructs without the loop on arm A (SL 2WJ, 3WJ, and 4WJ, Fig
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The primary antibodies were monoclonal mouse antibodies against TGF-[alpha] (1:500, Oncogene Science, Uniondale, NY) and EGFR (1: 20, Novocastra, Newcastle upon Tyne, United Kingdom)
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7 mg/mL^sup -1^ urea hydrogen peroxide and Tris buffer 0
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