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. At pH 4, the sample precipitated, and at pH 10, no endotherm was observed
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. Circular dichroism spectra of short, fixed-nucleus alanine helices
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. Hodge et al (50) were the first to report defective efferocytosis by alveolar macrophages taken from COPD patients
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. Sample preparation for neutron scatteringA batch of ~250 mg of HuBChE was dialyzed against 25 mM ammonium acetate
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. The thermodynamically irreversible step in disk denaturation is protein unfolding/dissociation and disk fusion that compensates for the reduction in the particle surface, leading to a high kinetic barrier for the disk denaturation (Gursky et al
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. 7) show reductions in intraresidue configurational entropy upon complexation, except for K22 and D53 in CD4, and C126, S365, and E429 in gp120
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. R] ABC reagent (avidin DH + biotinylated horseradish peroxidase/ PBS, 100 [micro]L) was added according to the direction of VECTASTAI[N
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. Using the hairpin ribozyme as a model system, we specifically address a key question in RNA folding-how do preformed secondary structure elements dictate the course of subsequent tertiary folding events? Currently, little is known about how secondary structural elements affect the ribozyme conformational distributions important in the course of folding
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.4, and the molarity of guanidinium hydrochloride was determined by refractive index as described by Pace (20)
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. RESULTS AND DISCUSSION Thermal and GndHCl-induced unfolded states All α-amylases investigated in this study consist of a monomeric structure with three domains
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.Identification of nanosecond-timescale internal motionThe presence of nanosecond-timescale motion can be directly identified from a plot of the normalized ratio of R^sub 1^ values measured at two magnetic fields (RT^sup n^^sub 1^) versus NOE (22)
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. In this procedure, the 50 µs data was first rebinned in 500-µs time bins, and rebinned data points lying above a threshold of 40 counts were identified (i
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.FRET histogram simulationsFRET histogram simulation routines were written in Matlab (The Math-Works, Natick, MA)
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