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. In native proteins containing both tyrosine and tryptophan residues, tyrosine emission is not commonly observed due to efficient energy transfer from tyrosine to neighboring tryptophan residues (Lakowicz, 1983)
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. The excess heat capacity functions were then analyzed using the software package Origin 7
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. Either we could not unambiguously determine the protein-concentration independence of the thermal midpoint from fluorescence measurements (see above, for the values, and Fig
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. On the other hand, the antigen recognized by Mab217 has not yet been characterized and could still act as an efferocytosis receptor, possibly through the recognition of PS
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. J Clin Invest 2002; 109:661-670(9) Hansen PR, Holm AM, Svendsen UG, et al
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. 5 mm outer diameter (received from Bruker Biospin, Karlsruhe, Germany) around a capillary MR tube of 1
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. Using 50-µs time bins and a threshold of 4 counts, we calculate the average burst duration to be ~280 µs
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. ([c] 2005 Book News, Inc
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. It is generally assumed that two distinct enzymes are involved in the intracellular degradation of N-acetylated proteins: the N-acetylated peptide hydrolase catalyzes the hydrolysis of the N-terminal amino acid residues from the N-acetylated proteins causing the release of acetylamino acids, which are finally hydrolyzed by ACYI (10,11)
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. Spectra were collected from 30°C to 98°C at intervals of 2°C with a spectral resolution of 4 cm^sup -1^ and 256 scans
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. It may also be possible that a smaller degree of subunit dissociation in part explains the sharp increase in the residual enzymatic activity, retained after freeze-thawing, with increasing protein concentration (14,16,29)
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. Therefore, depending on internal packing, it could be approximately estimated that the thick fiber may consist of over a dozen of laterally associated protofilaments
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. With these assumptions alone, the model of high-dimensional diffusion we have described is inevitable, and the timescales unreasonably long
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.We followed here a similar procedure to estimate errors on the conformational entropy changes upon complex formation for the sets of atoms that are involved in intermolecular interactions and/or show conformational changes (Table 1)
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.Feitel says the exercise led to a number of insights that were put into practice
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