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Despite the large amounts of ACYI that exist in mammalian kidney and liver, the reactivation of ACYI is particularly difficult in vitro (19-21), which suggests that the folding of ACYI in vivo might be assisted by the possible intracellular molecular chaperones and/or osmolytes
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Since conformational fluctuations are uncorrelated in a chain devoid of interactions, it is impossible to find large cavities (a > 5 [Angstrom]) within the interior of a freely rotating chain
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9, which plots the probabilities, P^sub oa^(r = U), of finding cavities of different sizes at the ensemble-averaged center-of-mass as a function of cavity radius a
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Beyond this length scale, all internal distances scale with sequence separation in accordance with the standard power law for proteins in a good solvent
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Excluded Volume Effects in Polymer Solutions as Explained by the Renormalization Group
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J Clin Invest 2002; 109:41-50(37) Odaka C, Mizuochi T, Yang J, et al
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Table 2 shows the required gradient field strengths for the respective voxel size and rf bandwidth
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We attributed this unfolding to cold denaturation of the S-S linked dimer (Gursky and Atkinson, 1996b), yet other studies suggested the importance of the oligomer dissociation (Osborne et al
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Several general predictions result: 1), intermediate subspaces of configurations will always be visited; 2), specific but nonnative interactions may be important in stabilizing these low-dimensional diffusive searches on the folding pathway, as well as native interactions; 3), sequential barriers will commonly be found, even in "two-state" proteins; 4), very early times will show characteristic departures from single-exponential kinetics; and 5), contributions of nonnative interactions to Φ-values and "Chevron plots" are calculable, and may be significant
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Put another way, the vast majority of the space covered by the energy landscape must actually be flat (on a scale of k^sub B^T) rather than funneled
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Manuscript approved for publication 2 March 2004 by the Scientific Editor
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Hence, quasi-docked and specifically-docked ribozyme species are readily distinguished in our diffusion spFRET experiments
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