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This work was supported by grants from the Howard Hughes Medical Institute, The John Simon Guggenheim Memorial Foundation, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, and Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro
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Steps in the pathway of the thermal unfolding of ribonuclecase A
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Pathways of protein folding
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Address reprint requests to Avadhesha Surolia, National Institute of Immunology, Aruna Asaf Ali Marg, New, Delhi-110067, India
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Hydrodynamic radii of native and denatured proteins measured by pulse field gradient NMR techniques
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The two cytoplasmic regions differed in their lipid contents and NMR lineshape characteristics-differences that are not detectable with whole-cell NMR techniques
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Given that early stage X
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For a random packing of noninteracting monodisperse hard spheres, these structures may form at volume fractions in the vicinity of 0
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Structural Transitions of transitions of deoxyribonucleic acid in aqueous solutions
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Friedman MJ, Berasi CC, Fox JM, et al
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. Investigation of the properties of these partially folded states using probes such as ANS or bis-ANS (which bind to exposed hydrophobic domains in proteins) was not possible due to the significant overlap between the emission spectra of these probes when bound to proteins and the excitation spectrum of the prosthetic group, FAD (data not shown)
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. the tertiary structure of EI^sup sc^ remained unaltered, as suggested by fluorescence and ANS-binding (Fig
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. Due to the high sensitivity of tryptophan fluorescence with respect to changes in the microenvironment of the intrinsic chromophores, various properties of the unfolded state can have a distinct effect on the λ^sub max^-values
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. Akke and co-workers (38) have also found larger ΔH^sup #^ values for residues that are part of regular secondary structure and smaller values for loop residues, in their study on the C-terminal domain of CaM
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