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There was no clear difference between N and A states in contrast to the 25% reduction of helix content and the 50% reduction of heat capacity change upon unfolding
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This is shown in Fig
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Remarkably, there is a 20% chance of linding a cavity of radius a = 12
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Analysis of the EV-limit ensembles suggests that the observations of local sequence-specific contacts are not incompatible with the observed power law behavior
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How random is a highly denatured protein
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Our results show that the secondary structure of EI^sup sc^ was not substantially altered, as suggested by FTIR experiments, but quenching experiments and proteolysis patterns suggest that there were small conformational changes upon binding of PEP; in addition, the thermal stability of EI^sup sc^ was slightly modified when any effector was added
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The addition of PEP alone slightly destabilized the protein (Table 3), suggesting that the conformational changes monitored by trypsin digestion and quenching experiments did not result in large thermodynamic variations
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In conclusion, the CaM:SEF2-1mp binding seems indeed distinct from other known protein:target binding modes
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1-fold activation of 2 nM PLCβ by 50 nM Gβγ (S
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We find that Gα(GDP) significantly decreased the volume change for the CPM-PLCβ-Gβγ association (Table 1)
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Wyllie Echeverria (1987) observed similar timing far parturition of black rockfish off north-central California, with a peak in February but with parturition occurring through May
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Q&A: Dawn Lepore, drugstore
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