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Apomyoglobin is one of the proteins most extensively studied in the protein folding research
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Although the fluorescence intensity signal exhibits this proportionality to the population of the microstates (i
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The observed red shift is caused by the fact that tryptophan residues are replaced from the less-polar interior of the protein to solvent exposed regions upon unfolding
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By assuming equilibrium conditions and an ANS activity in ice equal to the concentration in solution multiplied by a freeze concentration factor of 120 (12), one derives ANS dissociation constants in the range of 6-8 mM for WT, 2-4 mM for C3A/C26A, 2
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The resulting histogram can directly reveal conformational subpopulations that are averaged in conventional ensemble FRET measurements
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.* Reino: UHS enjoys the advantage of being able to do a lot of bench marking within our own enterprise
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. The I [arrow right] A transition, which was referred to as the dominant unfolding process above, began at around 70°C and the midpoint of the transition (T^sub 1/2^) occurred at ~84°C (see Table 1)
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. To our knowledge, this is the first demonstration of N-glycans affecting protein stability to such a huge extent, except for a report on a fucosylated peptide PMP-C (Pars interecrebralis major peptide C, a 36-residue peptide)
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. Above that temperature, the MSDs for HuBChE-D^sub 2^O are larger than those for Soman/HuBChE-D^sub 2^O, which remain in a quasiharmonic regime, as shown by their linear dependence on temperature (Fig
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. Increased protein backbone conformational entropy upon hydrophobic ligand binding
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.The maximum wetting forces for Sn-Ag-Cu-Bi solder in comparison with Sn-Pb solder are shown in Fig
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. When different τ^sub is^ values were tested, the lowest χ^sup 2^ values and hence the best overall fit was found for τ^sub is^ equal to 2
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.4 or in water-methanol mixtures at pH 7
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. The amino acid sequences of these proteins are comprised of tandem 11/22-residue repeats that have high propensity to form amphipathic α-helices with distinct charge distribution and large apolar faces that bind lipid (Segrest et al
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.1 mg/ml), when apoA-2 is largely self-associated (Osborne et al
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. Therefore the conserved A6-A11 SS-bond is expected to cause a significant topological constraint and bending of the A-chain
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. Additionally, the scan-angle was changed in cases of repeated scans
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. The transition from pelagic to benthic habitat in this species, i
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. The conformational sampling requires more time than for CD4
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