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. Both of these states are characterized by expanded hydrodynamic radii, loosening of tertiary structure, and preservation of secondary structure
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. Parameter A, which is a sensitive tool to reflect the position and shape of the Trp fluorescence spectrum, slightly decreased when the temperature increased to 64°C, and then an abrupt decrease was observed
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. The activity of EI is regulated by a monomer-dimer equilibrium, where the autophosphorylation requires the dimeric species (2-4)
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. The energies were calculated every 5 ps as reported earlier by Hsu and Bonvin, 2004, and therefore N is 16,001 when analyzing the 2-10 ns simulation segments
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. The peak position for the SL 3WJ remains in the low-FRET region of the histograms, independent of the Mg^sup 2+^ concentration, indicating that this ribozyme construct is incapable of folding to a compact structure (Fig
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. This led us to shift the excitation to a lower wavelength (270 nm) to minimize the contribution from Rayleigh scattering in the emission spectra
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.5 to 4 µM, and only KI was used as the quencher
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. In the triple-helix example that we looked at in detail, the nonnative hydrophobic strips that accelerate the final two-dimensional search are "activated" only when the helices themselves form, at least partially (by the formation of some native interactions) and when their axial rotational degrees of freedom align
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.Manuscript received July 8, 2003; accepted October 24, 2003
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