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The conformation changes cooperatively from native state to random coil between pH = 5 and 3
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This two-stage scheme is similar to that from the chemical unfolding/ refolding studies of ACYI (18,19), which also indicated that there existed a dimeric, aggregation-prone folding intermediate
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The glycosylation accounts for almost 6% (4
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Inasmuch as sequence-specific effects are present over five- to nine-residue stretches, it is conceivable that there are native-like local biases as well as default biases for conformations such as polyproline II (48)
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Solution structure of a protein denatured state and folding intermediate
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Dynamics on micro- to millisecond timescaleThree different approaches were used to determine which CaM residues in the CaM:SEF2-1mp complex are affected by conformational exchange
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In model colloidal systems the particles are generally spherical although they may have a limited polydispersity, and the system prepared so as to have well-defined interparticle forces
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This analysis can potentially allow us to discriminate between A-state monomers, protein oligomers, and larger aggregates
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As in the case of the Φ-value analysis, this experiment is also usually interpreted as giving information about the transition state (Oliveberg et al
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Bonvin** Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands; and [dagger] Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, Zurich, SwitzerlandSubmitted April 23, 2004, and accepted for publication September 20, 2004
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