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In this research, the sequential events in ACYI thermal unfolding were investigated by a combination of spectroscopic methods and the related resolution-enhancing techniques
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Since the frequency of the solvent-exposed secondary structures is smaller than the buried ones (44,45), the above sequential events could be interpreted as follows: the unfolding of the solvent-exposed β-sheet structures is the earliest event, followed by the formation of aggregates, and finally the unfolding of the other native structures
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This two-stage scheme is similar to that from the chemical unfolding/ refolding studies of ACYI (18,19), which also indicated that there existed a dimeric, aggregation-prone folding intermediate
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Folding of phage P22 coat protein monomers: kinetic and thermodynamic properties
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Biochem Biophys Res Commun 2004: 318: 32-37(52) Huynh ML, Malcolm KC, Kotaru C, et al
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One possibility was deactivated Gα-subunits that could displace Gβγ from PLCβ^sub 2^, but the addition of a large molar excess of Gα^sub i^(GDP)-subunits did not affect either the on- or off-rates
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Muscovite mica was purchased from Piano GmbH (Wetzlar, Germany)
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Other chemicals of suprapur or ultrapure grade were obtained from VWR (Merck GmbH, Darmstadt, Germany)
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It could be argued that cheese eaten on a regular basis adds balance to the diet and, hence, normality to our physiology
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The smallest mature female black rockfish we observed was 345 mm; all individuals were mature by 450 mm
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With fluorescence microscopy with B-exciting light, yellowish fluorescein coronas around the cells of the toxic species were observed, confirming a positive reaction
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In general, larger species of owls tend to produce pellets with lower proportions of bones
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NEAL WOODMAN1,*, CARLA J
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Items eligible for reimbursement include pain relievers, antacids, allergy and cold medicines, but not dietary supplements such as vitamins or toiletries such as toothpaste
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Stepka, who succeeds Jeff Kimmell, recently held a number of senior positions, including chief operating officer and vice president of marketing, at WorldRes, an online hotel reservation network
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"Initial Impact of a Medicaid Prior Authorization Program for NSAID Prescriptions
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8 percent were filled at independent drug stores, with mail order accounting for 17
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. 1 presents the progress of the spontaneous amyloidogenesis of insulin at 60° (left panel) and 70°C (right panel) monitored by time-lapse AFM
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