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5 and 3 M GdnHCl corresponded to expanded predissociated dimers and molten globule monomers, respectively
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3 B), and the second derivative spectra (Fig
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Refolding intermediate of guanidine hydrochloride denatured aminoacylase
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In the algorithm described above, steps 2-5 constitute a single trial move
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Analysis of main chain torsion angles in proteins: prediction of NMR coupling constants for native and random coil conformations
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3 µs and a repetition time of 2
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Modifications induced upon oligomerization in high-frequency, small amplitude/localized modes, which do lie within the sensitivity of our experimental window, might be rather small
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In total, 116 residues were analyzed using the PINATA method (22), which is particularly suited for proteins that exhibit extensive nanosecond-timescale internal motions
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Backbone dynamics of free barnase and its complex with barstar determined by 15N NMR relaxation study
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Interestingly, a lack of enhanced membrane binding was also observed for the nonspecific PLCβ-membrane association, suggesting that the enzyme packs efficiently with the membrane interface
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2, B and C; Fig
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The once-formed oligomers will remain after the temperature decrease, acting as early nucleation centers
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We need to articulate carefully what is meant in this context by "nonnative", for this term is sometimes used to refer to indiscriminate interactions
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Both positive and negative controls were included during each test
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1973) and therefore less likely to be present in egested pellets
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A similar finding was obtained in an earlier experiment (Phillips & Chau, 1988), which employed the same design, except that no monetary payoff was involved
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