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. This indicated that most of the Trp residues in ACYI were buried in the interior (Class I) or exposed to bonded water (Class II), and no Trp residues were in a highly hydrophobic (Class A and S) or fully solvent-exposed (Class III) microenvironment
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. 5 M GdnCl, as described previously (18)
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. The endotherm at pH 7 (Fig
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. Study of the "molten globule" intermediate state in protein folding by a hydrophobic fluorescent probe
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. Our results, however, can neither confirm nor exclude a modification in collective, low-frequency modes of HuBChE upon soman binding, which would give rise to changes in MSDs below the detection threshold of our technique (~5%)
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. Hence, there are four hinge regions in the CaM dimer
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. First, in contrast to lipid-free apoA-2, apoA-2 on DMPC disks shows no cold denaturation
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. This occurs through the application of excess shear during mix transfer as a result of the use of oversized or improperly operated centrifugal pumps or in the turbulence produced by sharp piping angles
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. Calorimetric denaturations were reversible in all cases
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. The data establish that every protein exhibits enhanced ANS fluorescence and therefore significant ice-induced ANS binding
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. DNA structure: cations in charge? Curr
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