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INTRODUCTION NMR allows detailed insights into molecular properties of liquids or solids, including, for instance, chemical structures and dynamics (1)
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Our results also highlight the ability of the diffusion spFRET method to resolve conformational subpopulations important during the course of RNA folding
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. Folding/unfolding can be followed by measurements of biological activity and/or by a variety of spectroscopic methods
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. At temperatures above 70°C, all the bands except those from the β-turn (1668 and 1674 cm^sup -1^) possessed similar melting curves, which indicated that a major conformational change occurred in this temperature range
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