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. Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein
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. This two-stage scheme is similar to that from the chemical unfolding/ refolding studies of ACYI (18,19), which also indicated that there existed a dimeric, aggregation-prone folding intermediate
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. , glycans attached to subunit X forming hydrogen bonds with residues of subunit X), whereas the rest are intersubunit interactions
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. org--California Dairy Research Foundation site on bioactives and probioticsReferences:(1) Baer DJ, et al
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. Reaching the transition region the intensity increases accompanied by a significant blue shift of ~8 nm
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. 4 ms was used for (180 µm)^sup 3^ voxel spectra, and TE = 6
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. (20), R^sub 2^ is measured for two settings of δ (we used 2δ^sub 1^ = 450 µs and 2δ^sub 2^ = 3
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. METHODSMaterialsBovine insulin (from Sigma Aldrich, Steinheim, Germany) was used without further purification
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. The reason for these differences is not known but may be related to technique, including antigen retrieval, types of antibodies used, or both
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. Therefore, the K^sub SV^ of 40 M^sup -1^ obtained for iodide quenching of GR in the presence of 7 M GdnHCl suggests that the Trp residues of the protein are significantly, if not completely, exposed to the solvent
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