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The proteins were redissolved in D^sub 2^O and centrifuged at 6000 × g for 10 min before use with a final pD of 8
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It is assumed that the chain swells uniformly vis-à-vis its theta state
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(31) Third, Mitchell and colleagues (28) recently demonstrated that PSR-deficient fibroblasts ingest and respond to apoptotic cells normally
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This is in contrast with stabilization by kosmotropic buffers, which decrease the free energy of the native state
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The most important studies on DNA-monocation binding are summarized in Table 1, which shows the activity sequences, methods used in research, and the corresponding citations in our list of references
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DCVJ was excited at 470 nm and ThT at 440 nm
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Similar increase in fluorescence quantum yield was evident in 50% w/w PEG (MW 3350; data not shown) as in 50% glycerol showing that increased viscosity is the main reason for the increased quantum yield
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We attributed this unfolding to cold denaturation of the S-S linked dimer (Gursky and Atkinson, 1996b), yet other studies suggested the importance of the oligomer dissociation (Osborne et al
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Challenges (all covered below) include precisely controlling lubricant mixing systems, maintaining thermal stability, choosing the appropriate cutting tools and removing the chips
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In addition, the conformer populations are obtained directly from the areas under each peak in the spFRET histograms, rather than being treated as adjustable parameters in the trFRET analysis
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