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. The transition curves of the amide I' hands were obtained according to a method described previously (28)
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. Since the frequency of the solvent-exposed secondary structures is smaller than the buried ones (44,45), the above sequential events could be interpreted as follows: the unfolding of the solvent-exposed β-sheet structures is the earliest event, followed by the formation of aggregates, and finally the unfolding of the other native structures
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. Each straight line in the phase diagram reflects an "all-or-none" process, and the joint position of two lines for a transition process indicates that an intermediate appeared at the corresponding temperature
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.de) to obtain the tertiary structure of the entire glycan (Fig
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. American Institute of Physics, New York
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. lib and b^sup /^) than for 255°C (Fig
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.The RT^sup n^^sub 1^ values for the CaM:SEF2-1mp complex are superimposed onto the S^sup 2^ contours in Fig
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. The presence of exchange between multiple-target sites and flexibility in the bound state leads to an important advantage over rigid binding, namely that the affinity (and specificity) is finely tuned
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. DCVJ should be especially useful for dynamic studies such as aggregation kinetics and detection of prefibrillar oligomers and can be used at acidic pH, which make this an attractive tool for in vitro studies
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