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. At 1 M GdnHCl, one thiol group of GR became accessible to modification with 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB), whereas no changes could be detected in the spectroscopic properties (fluorescence, circular dichroism) of the protein
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. Since spectral shift measurements showed a sharp transition between 1 and 3 M GdnHCl (Fig
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. All sequence-specific effects are restricted to a single local length scale, denoted as l^sub s^
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. We are developing methods to quantity the growth in the size of conformational space with chain length to test this prediction from scaling theories
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. We thank Maria Francisca Lozano for her involvement in the early stages of this work and Francisco N
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.ANS Fluorescence Detects Widespread Perturbations of Protein Tertiary Structure in IceGabellieri, EdiABSTRACT Freeze-induced perturbations of the protein native fold are poorly understood owing to the difficulty of monitoring their structure in ice
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. In C3A/C26A the elimination of the superficial C3-C26 disulfide bridge, linking the ends of strands β land β2, has little effect on the compactness of the protein core and, judging from the phosphorescence properties of W48 embedded in it (18), internal motions remain restrained
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. Thus, pressure methods allow for the estimation in the volume associated with the Gα^sub s^(GDP) deactivation of the PLCβ-Gβγ complex even though the change in interaction energy between PLCb and Gβγ caused by Gα^sub s^(GDP) could not be detected by spectroscopic atmospheric pressure measurements
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. Control samples (freshly cleaved mica, mica, and aqueous HCl-solution) were also investigated with AFM to exclude possible artifacts
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