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. The sensitivity of the ANS binding method to the tertiary structure of proteins in ice should find practical utility in devising safer freeze-drying protocols of pharmaceutical proteins and testing the effectiveness of various stabilizing/ destabilizing agents employed in protein formulations
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. For the 54% w/w solution, G' crosses G'' at ~2 Hz, a transition to an elastic solidlike response
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. The approach to generate these species in solution opens up the possibility to probe the process by a wide number of biochemical and biophysical techniques to provide insight into the mechanism of protein misfolding and misassembly
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. The Gβγ-subunits used in this study are geranylgeranylated, whereas Gα^sub s^-subunits are dually palmitoyated (Kleuss and Krause, 2003: Wedegaertner et al
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. This scan shows a sharp lipid transition at 24°C with a pretransition at 14°C that is characteristic of DMPC vesicles (curve 2 in Fig
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. BD=body depth; BL=body length;ED=eye diameter; HD=head depth; HL=head length; PAL=preanal length
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. A 96 well plate was used and 40 µl of both the standards and samples were pipetted into each well; 250 µl of DMB solution was added to each and the absorbance at 595 nm was determined using a microplate reader (Bio-Rad Ltd, Hemel Hempstead, UK)
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. Notes: The bold values indicate period-specificaverage monthly rates relative to rates in thepre-RP period
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- "I won't say it's widespread," says Mulford, "but there are enough examples to know that there is some confusion on reporting of these kinds of insurance proceeds
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. Molten globule monomers have been proposed as intermediates in the dissociation of some dimeric proteins, including human superoxide dismutase (Silva et al
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.2 mg/ml) similar to that used for the activity assay, except that the transitions occurred at a temperature ~2°C higher than the 1 mg/ml sample
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. The histogram of interresidue contacts can be plotted as a contact density map and the results are shown in the top row of Fig
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. Besides picosecond motions, all residues also experience internal motion with an effective correlation time of ~2
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. In summary, both the kinetic (Fig
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.01 kJ K^sup -1^ mol^sup -1^ (solid line in Fig
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. According to the estimates presented in Table 1, and as is evident from Figure 1, Type 1 RP had only negligible effects on the prescribing of other analgesics, but there was an increase in rates of opiate use after the introduction of Type 2 RP in the order of 492 days of therapy per month per 1,000 patients
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." Health Affairs 22: 49-59
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.Also weighing in was Rebecca Snead of the National Council of State Pharmacy Association Executives
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