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7 kcal mol^sup -1^ in terms of free energy of unfolding (27)
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This modeled structure has allowed us to explain the experimental conformational and thennodynamic features of the protein
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Steady-state ANS binding ANS binding was detected by collecting fluorescence spectra at different pH in the presence of 100 µM of dye
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or 350 nm, after excitation at 280 and 295 nm, with a scan rate of 30 K/h
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, 1995), "diffusion-collision" (Karplus and Weaver, 1976, 1994), "nucleation-condensation" (Fersht, 1999), and "topomer search" (Makarov and Plaxco, 2003) models
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A second feature is the potential for high cooperativity of structure in several simultaneous dimensions
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Casing CaseinEighty percent of the protein in milk is casein
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The energies were calculated every 5 ps as reported earlier by Hsu and Bonvin, 2004, and therefore N is 16,001 when analyzing the 2-10 ns simulation segments
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Elsevier, New York, NY
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We examined a series of six different ribozymes consisting of two-, three- and four-way junction variants, as well as corresponding constructs with one of the two loops removed
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, under conditions used for generating the FRET histograms in this article)
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One quarter of the ad-initiated doctor-patient conversations lead to diagnoses of treatable problems that might have gone undetected, the doctors report
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ResultsThe dependent variables were analyzed using two way mixed-model analyses of variance (ANOVA, Advice/No-Advice x Block) unless otherwise indicated
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- , Mg^sup 2+^ and PEP)
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. Although there were changes in the tertiary structure, EI^sup sc^ retained some, if not all, of its secondary structure, as shown by the increase (in absolute value) of the [Θ] at 222 nm (Fig
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. Thermodynamics in folding transition of DNA
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. ThT binds to oligomers and shows large amplitude in the slow second phase in response to formation of spherical aggregates and protofibrils, well in accordance with its reported amyloid specificity (Naiki et al
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. Amyloid fibrils in human insulinoma and islets of Langerhans of the diabetic cat are derived from a neuropeptide-like protein also present in normal islet cells
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. As an illustration, conformational entropy buildup curves as a function of block size with error bars derived from the block averaging procedure are presented for gp120 atom sets in Fig
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. The FRET efficiency histograms described in this article were generated by using a two-channel data collection mode to simultaneously record donor and acceptor signals as a function of time, with a time resolution of 0
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. All SL constructs show just a single peak in the FRET efficiency histograms, with no distinct docked peak, as expected (Fig
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