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This investigation aims to complement that initial study by addressing the following issues: i), establish whether the perturbation of protein structure reported for azurin is general to other proteins; ii), inquire on potential correlations between the susceptibility to the ice-induced perturbation and specific features of the macromolecule such as thermodynamic stability, internal flexibility, internal cavities/hydration, and covalent links between strands; and finally iii), test for a specific vulnerability of multimeric proteins to freeze damage that may be linked to the disruption of quaternary structure
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We interpret this such that aceturate experiences faster diffusion in the nucleus than in the macromolecule-rich animal cytoplasm and that, although there is no strong macromolecule-binding of aceturate molecules, they are nonetheless restricted in their translational and rotational motions, which would result in a short spin-spin relaxation time and a correspondingly large linewidth
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Relaxation time and diffusion NMR microscopy of single neurons
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The data points scatter around the line with k^sup -1^^sub ex^ [asymptotically =] 50 µs
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PLCβ contains the N-terminal PH domain of PLCβ^sub 2^ and the catalytic domain of PLCδ^sub 1^ (Wang et al
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, 2002) indicate similar rank order for the DMPC disk stability
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Error analysis of configurational entropiesThe configurational entropy differences between complex and free forms reported above were obtained from the entropy differences at 8 ns (the full trajectory length used for analysis)
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DISCUSSIONSufficient conformational sampling is a prerequisite for a reliable estimation of the conformational entropy
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i] = subsample count i, where i=1 to 3; and SS[W
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