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. Mutations in ACY1, the gene encoding aminoacylase 1, cause a novel inborn error of metabolism
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. It was suggested, with the help of some computational studies, that this enhanced stability of the tetramer is mainly due to glycosylation and some stabilizing ionic interactions at the oligomeric interface (18)
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. Stability of EI^sup sc^ as monitored by DSC and thermal denaturations From the spectroscopic results above, it can be concluded that EI^sup sc^ acquired a folded conformation around physiological pH, and that this structure was basically unaltered (exeept for changes in the ellipticity, Fig
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. The fluorescence profiles of all seven proteins in ice, at -13°C, are compared in Fig
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. Glycoforms modify the dynamic stability and functional activity of an enzyme
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. The observed concentration-dependent behavior suggests that the influence of this hydration layer on viscosity decreases with increasing concentration
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. Arrhenius analysis reveals two kinetic phases, a slower phase with E^sub a,slow^ = 60 kcal/mol and a faster phase with E^sub a,fast^ = 22 kcal/mol
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. The insulin aggregation is promoted under conditions favoring partly destabilized monomers and dimers, such as low pH, high temperature, and contact with hydrophobic media (e
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. major (20 April, 1995) could react with the antibodies obtained as clearly as the recently collected eggs of P
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. To further evaluate the contribution of solvent-induced shifts, we examined a model duplex DNA molecule whose structure is not expected to change significantly with Mg^sup 2+^ concentration changes in the 0
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. Accordingly DNA values were significantly greater at day 14 for pellets maintained in DMEM/ITS+ATGF-[beta]1 compared with DMEM/10%FCS (p Morphology and immunolocalisation
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. Here we applied this method to analyze delta and gamma dispersions for protein solutions
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. Third, these increased fluctuations could certainly lead to the occasional close approach of distal amino acids
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. The β-mercaptocthunol was from BioRad (Hercules
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. However, the Fold-X analysis of the modeled EIC^sup sc^ dimer showed differences in the stability and in the interaction energy when compared to those of EIC^sup tt^ (11)
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. For the irreversible unfolding α-amylases studied here, transition temperatures and magnitudes of red shifts are strongly depending on heating rates
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.RESULTS AND DISCUSSIONThe maximum wetting forces as a function of solder bath temperatures for Cu substrate are shown for three fluxes in Fig
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.Structural interpretation of the nanosecond-timescale internal motionThe dimeric CaM:SEF2-1mp complex can in principle show internal modes of motion involving either the individual N- and C- terminal domains and/or the CaM monomers as a whole
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. 4 C shows the data recorded in the first and second jump from 25 to 85°C
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.Under physiological conditions, insulin is a hexamer, being able to bind two or four Zn^sup 2+^ ions (e
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