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. The free energy of unfolding calculated for rSBA and gSBA as per LEM model are 41
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. Given the equivalence between the EV limit and highly denatured states, our ability to simulate conformational ensembles in the EV limit, with full atomic detail, has direct bearing on the development of an accurate physical picture of conformations accessible to denatured proteins
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. 2 C and 3 A) (Table 3)
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. The stability of these mutants is ~3 kcal/mole lower relatively to the WT (20-22), and the internal flexibility of the polypeptide is considerably larger, particularly with I7S and F110S (18)
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.For studies of conformational exchange it is important to ascertain whether the exchange is in the fast or slow exchange limit
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